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pTol2-nbt-mTagBFP2-P2A-mScarlet

Plasmid

#196115

Purpose

zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-mTagBFP2-P2A-mScarlet

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-BFP

Plasmid

#137131

Purpose

Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-PIF-SOS2cat

Plasmid

#50850

Purpose

Optogenetic Ras activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Inserts

SOS2cat (SOS2 Human)
PIF6 1-100

Use

Lentiviral

Tags

TagBFP

Expression

Mutation

residues 566–1049

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-p65AD-BFP

Plasmid

#46913

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-p65AD-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

2xNLS, BFP, and VP64 domain

Expression

Mammalian

Mutation

Promoter

LTR and PGK

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

Plasmid

#213119

Purpose

Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-hSyn-NCreIntN-T2A-mTagBFP2

Plasmid

#160502

Purpose

Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTP341 pHAGE2 CMV TagBFP-3xFLAG

Plasmid

#199391

Purpose

Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

TagBFP-3xFLAG

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Bhlhe40-3xFLAG-IRES-BFP

Plasmid

#117263

Purpose

Retroviral overexpression of Bhlhe40 with a 3xFLAG

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Bhlhe40 (Bhlhe40 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAF0690 Ef1a-Kp03_attB-BFP acceptor

Plasmid

#193473

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pscAAV-CBh-tagBFP2-WPRE3-enSV40pA

Plasmid

#194246

Purpose

Expresses tagBFP2

Depositor

Michael J Fox Foundation MJFF

Insert

tagBFP2

Use

AAV

Tags

Expression

Mutation

Promoter

CBh

Available since

May 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_UAS-tTS-P2A-GFP_PGK-BFP

Plasmid

#133808

Purpose

inducible expression of tTS and GFP with BFP marker in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

tTS-P2A-EGFP-PGK-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Expression

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mTagBFP2-2A-FLAG-ntcGAS

Plasmid

#102603

Purpose

Lentivector to express Flag-tagged and shRNA-resistant cGAS and mTagBFP

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-T2A-mTagBFP2

Plasmid

#160500

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING 368STOP

Plasmid

#102590

Purpose

Lentivector to express STING delta 368 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

delta 368

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-moxBFP-IRES-Flpo

Plasmid

#194977

Purpose

AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP, Flpo

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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