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Addgene

pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP
(Plasmid #213119)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213119 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTwist SFFV
  • Backbone manufacturer
    Twist
  • Backbone size w/o insert (bp) 5683
  • Total vector size (bp) 8527
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ActA(1-584)-BFP-FKBP
  • Species
    Listeria monocytogenes
  • Insert Size (bp)
    2844
  • Entrez Gene
    actA (a.k.a. lmo0204)
  • Promoter SFFV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Purchased from Twist Bioscience

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP was a gift from Scott Coyle (Addgene plasmid # 213119 ; http://n2t.net/addgene:213119 ; RRID:Addgene_213119)
  • For your References section:

    A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design. Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM. Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007. 10.1016/j.cell.2023.12.007 PubMed 38181787