pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP
(Plasmid
#213119)
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PurposeExpression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTwist SFFV
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Backbone manufacturerTwist
- Backbone size w/o insert (bp) 5683
- Total vector size (bp) 8527
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameActA(1-584)-BFP-FKBP
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SpeciesListeria monocytogenes
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Insert Size (bp)2844
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Entrez GeneactA (a.k.a. lmo0204)
- Promoter SFFV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPurchased from Twist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP was a gift from Scott Coyle (Addgene plasmid # 213119 ; http://n2t.net/addgene:213119 ; RRID:Addgene_213119) -
For your References section:
A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design. Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM. Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007. 10.1016/j.cell.2023.12.007 PubMed 38181787