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Showing: 8621 - 8640 of 18167 results

  3. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86232-plasmid-map-sequence-id-177242

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  4. dxCas(3.6)-VPR

    Plasmid
    #108388
    Purpose
    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Article
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Insert
    dxCas(3.6)-VPR
    Use
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Promoter
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108388-plasmid-map-sequence-id-207184

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  12. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  16. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127359-plasmid-map-sequence-id-246306

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  18. pJS288

    Plasmid
    #200714
    Purpose
    To express yeast Hxt1 hexose transporter in yeast
    Depositor
    Jens Boch
    Article
    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi
    Inserts
    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    domesticated for MoClo cloning (removal of intern…
    Promoter
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    200714-plasmid-map-sequence-id-404927

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Showing: 8621 - 8640 of 18167 results