pCAG-GST-hFIP200(1363-1370Mut)-MBP
(Plasmid
#190038)
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PurposeExpression of FIP200 mutant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFIP200
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Alt nameGST-TEVsite-hFIP200 (1363 RDKDLIES 1370 to GSSGGSSG)-MBP
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SpeciesH. sapiens (human)
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Mutation1363 RDKDLIES 1370 to GSSGGSSG
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Entrez GeneRB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
- Promoter CMV
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Tags
/ Fusion Proteins
- GST (N terminal on insert)
- MBP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer ctggcaagccacgtttggt (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-GST-hFIP200(1363-1370Mut)-MBP was a gift from James Hurley (Addgene plasmid # 190038 ; http://n2t.net/addgene:190038 ; RRID:Addgene_190038) -
For your References section:
The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment. Shi X, Chang C, Yokom AL, Jensen LE, Hurley JH. Elife. 2020 Aug 10;9. pii: 59099. doi: 10.7554/eLife.59099. 10.7554/eLife.59099 PubMed 32773036
