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Plasmid#148038PurposeInsect Expression of DmPAN2-del486-493GSG-dsRNAresDepositorInsertDmPAN2-del486-493GSG-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-del975-1031GSSG-dsRNAres_Y
Plasmid#148039PurposeInsect Expression of DmPAN2-del975-1031-GSSG-dsRNAresDepositorInsertDmPAN2-del975-1031-GSSG-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-del1047-1063GSG-dsRNAres_Y
Plasmid#148040PurposeInsect Expression of DmPAN2-del1047-1063GSG-dsRNAresDepositorInsertDmPAN2-del1047-1063GSG-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-F99EG132EL133E-dsRNAres_Y
Plasmid#148042PurposeInsect Expression of DmPAN2-F99EG132EL133E-dsRNAresDepositorInsertDmPAN2-F99EG132EL133E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-F550EM554EK557E-dsRNAres_Y
Plasmid#148043PurposeBacterial Expression of DmPAN2-F550EM554EK557E-dsRNAresDepositorInsertDmPAN2-F550EM554EK557E-dsRNAres (PAN2 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-CG32016-E_T
Plasmid#147583PurposeInsect Expression of DmCup_1-417-CG32016-EDepositorInsertDmCup_1-417-CG32016-E (cup Fly)
UseTagsExpressionInsectMutation5 silent and three no silent mutations H117R, N14…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-DmCup_417-1133_T
Plasmid#147589PurposeInsect Expression of DmeIF4Ga_578-650-DmCup_417-1133DepositorInsertDmeIF4Ga_578-650-DmCup_417-1133 (eIF4G1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I
Plasmid#146577PurposeInsect Expression of DmeIF4GaDepositorInsertDmeIF4Ga (eIF4G1 Fly)
UseTagsExpressionInsectMutationone non silent mutation K340R compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_578-634-GB1_AG
Plasmid#148744PurposeBacterial Expression of DmeIF4G_578-634DepositorInsertDmeIF4G_578-634 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPT-mEGFP
Plasmid#190606PurposeObtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)DepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationa deletion was created in mCherry CDS impairing i…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z
Plasmid#148160PurposeInsect Expression of Dm4E-T_1-511-LLFWKtoAAAAADepositorInsertDm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
UseTagsExpressionInsectMutationalias: 4E-T; one non silent mutation L127S, annot…PromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1-p75
Plasmid#182945PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTRDepositorUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1
Plasmid#182944PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTRDepositorInsertMNTS-1 (NTF3 Synthetic, Human, Homo sapiens)
UseLentiviralTagsExpressionMammalianMutationThis is the Human NT-3 with mutations on the matu…PromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-GB1_Z
Plasmid#148180PurposeBacterial Expression of DmThor_50-83DepositorInsertDmThor_50-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y
Plasmid#148075PurposeInsect Expression of DmPAN2-F1103A-dsRNAresDepositorInsertDmPAN2-F1103A-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-660-GB1_AD
Plasmid#148506PurposeBacterial Expression of DmeIF4G_601-660DepositorInsertDmeIF4G_601-660 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y
Plasmid#148046PurposeBacterial Expression of DmPAN2-K1124EY1127EK1131E-dsRNAresDepositorInsertDmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.4.hSyn.H2B.RFP
Plasmid#170387PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.3.hSyn.H2B.RFP
Plasmid#170370PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only