Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 15,360 results for: grna

Showing: 8021 - 8040 of 15360 results

  1. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

    Enlarge

  2. pXPR_045

    Plasmid
    #107144
    Purpose
    cloning of a sgRNA
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107144-plasmid-map-sequence-id-205294

    Enlarge

  3. L4440gtwy

    Plasmid
    #11344
    Depositor
    Guy Caldwell
    Insert
    gateway cassette
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11344-plasmid-map-sequence-id-384093

    Enlarge

  4. pT076

    Plasmid
    #137880
    Purpose
    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP
    Depositor
    Lindy Barrett
    Article
    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.
    Insert
    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    137880-plasmid-map-sequence-id-271983

    Enlarge

  5. pT077

    Plasmid
    #137879
    Purpose
    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP
    Depositor
    Lindy Barrett
    Article
    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.
    Insert
    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    137879-plasmid-map-sequence-id-271982

    Enlarge

  7. JD633

    Plasmid
    #160393
    Purpose
    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning
    Depositor
    Jorge Dubcovsky
    Article
    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103
    Inserts
    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    Use
    Binary vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160393-plasmid-map-sequence-id-456253

    Enlarge

  8. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDPGH023
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

    Enlarge

  11. SiC-V1-Scr

    Plasmid
    #133042
    Purpose
    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133042-plasmid-map-sequence-id-264423

    Enlarge

  14. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101165-plasmid-map-sequence-id-199321

    Enlarge

  20. pX330-EN479

    Plasmid
    #86234
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse rosa26 locus
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86234-plasmid-map-sequence-id-168051

    Enlarge

Showing: 8021 - 8040 of 15360 results