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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneL4440
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Backbone manufacturerL4440 is available at Addgene
- Backbone size w/o insert (bp) 2790
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Vector typeWorm Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsThis vector is propagated in E. coli strain DB3.1, due to the requirement to circumvent the lethality of the inherent ccdB gene until an insert is recombined into the Gateway cassette.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegateway cassette
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer L4440 primer (AGCGAGTCAGTGAGCGAG) (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Andrew Fire created the vector backbone L4440
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gateway-modified RNAi feeding vector L4440. Use this vector for Gateway cloning of an RNAi target sequence. A Gateway recombinational cassette was placed into the EcoRV site of vector L4440 to generate this modified plasmid. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.)
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L4440gtwy was a gift from Guy Caldwell (Addgene plasmid # 11344 ; http://n2t.net/addgene:11344 ; RRID:Addgene_11344)