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pcDNA3.1_hE2F7mg

Plasmid

#73073

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Use

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-nEF-dCas9

Plasmid

#106430

Purpose

Expresses SpdCas9 from the nEF promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

nEF-dCas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Mutation

Promoter

nEF

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pIDS-LSD1fl

Plasmid

#109157

Purpose

Encodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length LSD1, sequence-optimized for insect cells (KDM1A Human)

Use

Tags

Expression

Insect

Mutation

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-tdTomato

Plasmid

#28305

Purpose

Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV5

Insert

ArchT-tdTomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIgkc

Plasmid

#127157

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Igkc (Igkc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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AbVec2.1-mIglc2

Plasmid

#127156

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Iglc2 (Iglc2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-FLucStop

Plasmid

#177617

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

In-frame stop codon added between FLuc and FSE

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Mutation

Promoter

CMVc

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGK-HELLO-T2A-Cre LV

Plasmid

#202003

Purpose

Generates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP mice

Depositor

Nikhil Joshi

Article

Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

Insert

HELLO-T2A-Cre

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

pGK

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CD-MPR-GFP-RUSH

Plasmid

#202797

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

Plasmid

#165456

Purpose

Inducible ZEB2 expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

ZEB2 (ZEB2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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