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Plasmid#184878Purposeexpresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutantDepositorInsertpfQC-cyclase dead
UseTagsHis tagExpressionBacterialMutationF77A & Q79APromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(I154F)-HA
Plasmid#14988DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationchanged isoleucine 154 to phenylalaninePromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(T133A)-HA
Plasmid#14989DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationchanged threonine 133 to alaninePromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(T133E)-HA
Plasmid#14990DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationchanged threonine 133 to glutamic acidPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(AscI)
Plasmid#15404DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutation700 bp insert can be released by digestion with r…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>m2a(silent)-srt-his
Plasmid#124807PurposeHDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.DepositorInsertMurine IgG2a (Fc silent)
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationL234A/L235A/N297APromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)
Plasmid#162880PurposeExpression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFPDepositorInsertPhosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)
Plasmid#162877PurposeExpression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALMDepositorInsertpleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
UseTagsmEos2ExpressionMammalianMutationInsert consists of AA1-170PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>Fab-srt-his
Plasmid#124810PurposeHDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.DepositorInsertrat IgG2a Hinge-G4S-Sortag-Histag
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterialMutationPromoterpUCAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(NcoI/EcoRI)
Plasmid#15405DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationinsert can be released by digestion with REs NcoI…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(2kb, distal)
Plasmid#14981DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationincludes distal fragment of human frataxin promot…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
FXN_Halo_N_allele
Plasmid#178141PurposeDonor vector for endogenous tagging of human FXN at the N-terminus with halotagInsertHalotag (FXN Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)
Plasmid#162878PurposeExpression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALMDepositorInsertPhosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p(bhsp68-Cas9)
Plasmid#65959PurposeTribolium basal heat shock promoter driving Cas9DepositorInsertCas9
UseCRISPRTagsnuclear localization sequenceExpressionInsectMutationPromoterbhspAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(2,5kb)
Plasmid#14980DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationstart codon for human frataxin gene has been muta…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(1,3kb)
Plasmid#14979DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationstart codon for human frataxin gene has been muta…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(I154F)(AA88)-HA
Plasmid#15403DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(1kb)
Plasmid#14978DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationstart codon for human frataxin gene has been muta…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-hFX(I154F)-HA
Plasmid#14992DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSIREN-RetroQ-BPTF-sh28
Plasmid#73669PurposepSIREN-RetroQ vector containing shRNA sequence to BPTFDepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits