Addgene

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pCDNAIIIB rhoA WT

Plasmid

#61631

Purpose

mammalian expression of rhoA

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad2 (1-456)

Plasmid

#14958

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-456 (tail deletion)

Promoter

CMV

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Use

Tags

HA, lifeact, and p2a

Expression

Plant

Mutation

Promoter

35S

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA RBD-GFP11

Plasmid

#133069

Purpose

Expresses RBD domain with tripartite split GFP11 (M4)

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

RBD (Rhotekin-Rho Binding Domain)

Use

Tags

GFP11 (tripartite split-GFP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB522

Plasmid

#74864

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Expression

Mutation

H134R

Promoter

GfaABC1D

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00311

Plasmid

#34436

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9786 (hb Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pLib6.4-Cquin_596

Plasmid

#176655

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pETpRha-alpha-SkTm

Plasmid

#72929

Purpose

Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha skeletal Tropomyosin

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaB

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEGFP-NLS

Plasmid

#176681

Purpose

Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5aa

Plasmid

#109648

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5aa (rab5aa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5ab

Plasmid

#109649

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5ab (rab5ab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1-WT 6KQ

Plasmid

#17559

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KQ (Foxo1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
mCherry-E2

Plasmid

#175242

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-HDD

Plasmid

#74991

Purpose

Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

HDD = homeodomain deleted

Promoter

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00308

Plasmid

#34433

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5204 (CG5204 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR-v1-sgMPC2_7

Plasmid

#163457

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 7 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMPC2_9

Plasmid

#163458

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15485_Hypa-ABE8e

Plasmid

#174123

Purpose

Expresses ABE8e fused with Hypa-SpCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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ATF4 10: 5’ATF4.uORF1.GFP

Plasmid

#21857

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF1 (uORF) of ATF4 fused to EGFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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