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Plasmid#145795PurposemPing Yeast Transposition AssayDepositorInsertPong TPase L418A, L420A
UseTagsExpressionYeastMutationL418A, L420APromoterAvailable sinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-sgRNA expression vector
Plasmid#51132PurposeFor in vitro transcription of sgRNA from the T7 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterT7Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYTipi
Plasmid#53283PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p6642 MSCV-P C-FlagHA 6bE7-Kozak
Plasmid#35020DepositorInsertHPV6b E7 (E7 HPV 6b)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
HcKCR2_mCherry_pcDNA3.1
Plasmid#177337PurposeExpresses KCR2 in mammalian cellsDepositorInsertHcKCR2
UseTagsmCherryExpressionMammalianMutationmammalian codon-adaptedPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis S198A
Plasmid#213605PurposeExpresses PLA2G15-6xHis S198A in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
UseTags6xHisExpressionMammalianMutationChanged serine 198 to alaninePromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUB_smFLAG_KDM5B_MS2
Plasmid#81084PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_KDM5B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc wt MBS1-4
Plasmid#16564DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
neurod1:CaMPARI
Plasmid#173479PurposeCaMPARI is driven by -5kb neurod1 promoter.DepositorInsertCaMPARI
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
UseTagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6644 MSCV-P C-Flag HA 45E7-Kozak
Plasmid#35135DepositorInsertHPV45 E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA iRFP713
Plasmid#167908PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6656 MSCV-P C-Flag HA 25E7-Kozak
Plasmid#35149DepositorInsertHPV25 E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMpGE013
Plasmid#108681PurposeAll-in-one genome editing vector in M. polymorpha (hygromycin resistant)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p6652 MSCV-P C-Flag HA 8E7-Kozak
Plasmid#35145DepositorInsertHPV8 E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
p6653 MSCV-P C-Flag HA 92E7-Kozak
Plasmid#35146DepositorInsertHPV92 E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only