Real-time quantification of single RNA translation dynamics in living cells.
Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899. Epub 2016 May 5.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
81082 | pUB_smFLAG_H2B_MS2 | Translation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG). |
81083 | pUB_smFLAG_ActB_MS2 | Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG). |
81084 | pUB_smFLAG_KDM5B_MS2 | Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG). |
81085 | pUB_smHA_KDM5B_MS2 | Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA). |