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Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_PURO
Plasmid#155295PurposeLentiviral vector to generate hACE2 stable expressing cell lineDepositorInserthACE2 (ACE2 Human)
UseLentiviralTagsC9ExpressionMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Neurofascin-186-HA
Plasmid#31061DepositorInsertNeurofascin (Nfasc Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64
Plasmid#85424PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-7_synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-MCS
Plasmid#185594PurposeControl lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
UseTagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Neo-Myr-Flag-DEST
Plasmid#15300Purposea Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORFDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Abl-His6-FLAG
Plasmid#52684Purposeexpression of Abl in mammalian cellsDepositorInsertABL1 (Abl1 Mouse)
UseTagsFLAG and His6ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300
Plasmid#191764PurposeExpression of EGFP fused to core p300 histone acetyltransferaseDepositorInsertFRB-EGFP-p300 core (EP300 Human)
UseTagsEGFP (N-terminal of p300 core) and FRB (N-termina…ExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PDGFRB
Plasmid#116770PurposeLentiviral expression of PDGFRBDepositorInsertPDGFRB (PDGFRB Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-Rabin8
Plasmid#118070PurposeExpresses Flag-tagged human Rabin8 in mammalian cellsDepositorInserthuman Rabin8 (RAB3IP Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142
Plasmid#69294PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCAS9-mCherry empty
Plasmid#80975PurposeCas9, mCherry, and sgRNA expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human pIgR(SC)
Plasmid#158216PurposeExpress the ectdomain (SC) of human pIgRDepositorInsertPIGR (PIGR Human)
UseTags8×hisExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS
Plasmid#187588PurposeExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cellsDepositorInsertNLS
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3_MERS_D12
Plasmid#170448PurposeExpress MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.DepositorInsertMERS spike D12 (S MERS)
UseTagsExpressionMammalianMutationlast 12aa deletion in c-terminal tailPromoterCMVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only