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Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its Phosphatase Activity and Modulates Ligand Specificity.

Burmeister BT, Wang L, Gold MG, Skidgel RA, O'Bryan JP, Carnegie GK
J Biol Chem. 2015 May 8;290(19):12058-67. doi: 10.1074/jbc.M115.642983. Epub 2015 Mar 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67571pEGFP-FLAG-AKAP-LbcExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells
67575pGEX-4T-1-Shp2 SH2sExpresses GST-tagged Shp2 SH2 domains in bacteria
67577pGEX-4T-1-Shp2 T7DA/S189D SH2sExpresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria

Antibodies from Article