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Addgene
Showing: 7421 - 7440 of 13941 results

  1. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

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  2. pcDNA3.1-hRIPK1-Flag

    Plasmid
    #112487
    Purpose
    Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112487-plasmid-map-sequence-id-214532

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  3. P37m

    Plasmid
    #120947
    Purpose
    MoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pRpa* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120947-plasmid-map-sequence-id-236378

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  4. P38m

    Plasmid
    #120948
    Purpose
    MoClo golden gate assembly AB part for pTra* (improved pTra from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pTra* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120948-plasmid-map-sequence-id-236376

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  5. C22m

    Plasmid
    #120963
    Purpose
    MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mSpinach
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120963-plasmid-map-sequence-id-236357

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  6. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

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  7. C90m

    Plasmid
    #120998
    Purpose
    MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    NahRsfGFP fusion
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120998-plasmid-map-sequence-id-236128

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  8. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

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  9. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

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  10. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

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  11. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

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  12. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

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  13. DsRed-CPI-17-T38E-NLS

    Plasmid
    #100790
    Purpose
    Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100790-plasmid-map-sequence-id-193221

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  14. pOpen-T7gene5

    Plasmid
    #165534
    Purpose
    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    T7 DNA Polymerase (unmodified)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165534-plasmid-map-sequence-id-339124

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  15. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  16. SIRT6

    Plasmid
    #28271
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. PP-HSP90 (3Q5J)

    Plasmid
    #28281
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. PP-G6PI

    Plasmid
    #28283
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-G6PI (PF14_0341 Plasmodium falciparum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    28283-plasmid-map-sequence-id-235428

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  19. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

    Plasmid
    #86455
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86455-plasmid-map-sequence-id-171155

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  20. mEmerald-Nesprin3-C-18

    Plasmid
    #54203
    Purpose
    Localization: Nuclear Membrane , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    54203-plasmid-map-sequence-id-247990

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Showing: 7421 - 7440 of 13941 results