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Plasmid#190106PurposeExpresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE useDepositorInsertMMLV-RT(delta RNAseH)
UseTagsbpNLSExpressionMammalianMutationmutations from RT in PE2 and truncation of RNAse …PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9
Plasmid#80425PurposeUsed for in vitro transcription of SpCas9 mRNA.DepositorInsertSpCas9
UseCRISPRTagsnoneExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-N–dSpCas9
Plasmid#157835Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertbpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg14x(MS2) MUC4.1
Plasmid#101153PurposegRNA with 14 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-4mtGCaMP6f-DIO
Plasmid#179529PurposeCre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoterDepositorInsert4mtGCaMP6f
UseAAVTags4x Cox8 targeting sequenceExpressionMutationPromoterEf1aAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DEST PINK1-V5 WT
Plasmid#13320DepositorInsertPINK1 (PINK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-3
Plasmid#27289DepositorInsertintegrin beta 3 (ITGB3 Human)
UseTagsHis and MycExpressionMammalianMutationn/aPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Dest
Plasmid#74281PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-D
Plasmid#38133DepositorInsertMITF-D (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRY2FL(deltaNLS)-mCherryN1
Plasmid#26871PurposeMammalian expression of cytoplasmic CRY2-mCherryDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationNLS sequences deletedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.4_17G6-1 light chain
Plasmid#198250Purposerecombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1DepositorInsertlight chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4
Plasmid#21156DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
sg2.02 16x(MS2) MUC4.1
Plasmid#101154PurposegRNA with 16 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (WT)
Plasmid#73963PurposeSTAG2 cDNA (CCDS43990) wild typeDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationwild typePromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
UseTagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIB1(deltaNLS)-pmGFP
Plasmid#28240PurposeExpresses CIB1 fused to plasma membrane-localized EGFPDepositorInsertCIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV1 for Split-PE, SpCas9 nickase (PKC1001)
Plasmid#190111PurposeAAV1 for expression of nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
UseAAVTagsbpNLSExpressionMutationH840APromoterEF1aAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX-evoCas9
Plasmid#107550PurposeExpresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cellsDepositorInsertHumanized S. pyogenes Cas9
UseCRISPRTags3XFLAGExpressionMammalianMutationM495V/Y515N/K526E/R661QPromoterCBhAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/BFP Dest
Plasmid#74282PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only