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Plasmid#72590PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGDF8 donor CFP-hDF
Plasmid#72591PurposePlasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX68
Plasmid#46857PurposeOP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCEDepositorInsertattB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)
UseTagsExpressionMammalianMutationp53 A138VPromoterAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT
Plasmid#111072PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.5 AB (361-545, Δ395-511)/pET28HMT
Plasmid#111267PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.5 AB (361-545, Δ395-511)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1
Plasmid#111460PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/I346A, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1
Plasmid#111461PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/I346D, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1
Plasmid#111462PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/S536A/L540A, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1
Plasmid#111497PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/S536D/L540D, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
ARMET-bio-His
Plasmid#52026PurposeExpresses full-length Mesencephalic astrocyte-derived neurotrophic factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertARMET (MANF Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40
Plasmid#101060PurposeAAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA
Plasmid#197044PurposeIn situ cell-surface proteome extraction by extracellular labeling (iPEEL)DepositorInsertspCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA
UseTagsHA, mycExpressionMammalianMutationPromoterpCAGAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-G2019S
Plasmid#25045DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationGlycine 2019 mutated to SerinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP-EF1a-mCherry
Plasmid#115782PurposeSox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.DepositorInsertsmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
EF1alpha promoter driven constitutive mCherry
UseLentiviralTagsEF1α promoter fused to mCherry and cfos promoter …ExpressionMutationPromoterSOX10 Multiple Species Conserved enhancer element…Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai65(RCFL-tdT) targeting vector
Plasmid#61577PurposeTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertpCAG-FSF-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-sRGECO
Plasmid#137126PurposeIntersectional viral expression of sRGECO in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE2
Plasmid#87085PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApolipoprotein E2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ai66(RCRL-tdT) targeting vector
Plasmid#61578PurposeTarget a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertCAG-RSR-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_VIP1.0
Plasmid#208681PurposeExpresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neuronsDepositorInsertGPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FLEX-EGFP-WPRE-bGHpA
Plasmid#65449PurposeCan be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent mannerDepositorInsertEGFP
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only