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pLVX-UBE3B-HA-IRES-Puro

Plasmid

#107346

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR223-BMRF1(EA-D)

Plasmid

#195807

Purpose

Gateway Entry Clone of Epstein-Barr virus BMRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BMRF1 (BMRF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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UBXD1-GFP

Plasmid

#86464

Purpose

Expression of human UBXD1 with C-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

UBXD1 (UBXN6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 ∆24-mCherry

Plasmid

#80271

Purpose

mammalian expression plasmid for Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 R443A

Plasmid

#162652

Purpose

Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Use

Tags

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pMRX-IPU-FLAG-NEK9

Plasmid

#168269

Purpose

Expresses NEK9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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Puro-iNgn3

Plasmid

#75337

Purpose

doxycycline inducible Ngn3 over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

neurogenin 3 (Neurog3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT5_HA

Plasmid

#24483

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Sirtuin 5 (SIRT5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4A

Plasmid

#41914

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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Kindlin

Plasmid

#186135

Purpose

For expression of recombinant kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin3

Plasmid

#41913

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

Cullin 3 (CUL3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4B

Plasmid

#41915

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4B (CUL4B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28A

Plasmid

#51371

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRDA_722

Plasmid

#216077

Purpose

CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Type

Empty backbone

Use

CRISPR and Lentiviral; Destination

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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GB-Dcp1b

Plasmid

#153979

Purpose

Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1b (DCP1B Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIID-K43M

Plasmid

#186830

Purpose

Expresses FLAG-CaMKIID-K43M in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2d (Camk2d Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 43 to Methionine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Incenp wt

Plasmid

#108487

Purpose

expression of vsv-INCENP wt-mCherry

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Use

Tags

VSV and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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