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Plasmid#190720PurposeFor microglia differentiation from iPSCDepositorInsertMAFB-IRF8-CEBPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
EB3-tdTomato
Plasmid#50708Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTsDepositorInsertEB3 (MAPRE3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-BRCA1
Plasmid#71117Purposemammalian expression of Flag-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV/TOAvailable sinceNov. 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pY108 (lenti-AsCpf1)
Plasmid#84739PurposeLenti virus delivery of AsCpf1 and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2AExpressionMutationPromoterEFSAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY109 (lenti-LbCpf1)
Plasmid#84740PurposeLenti virus delivery of LbCpf1 and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2AExpressionMutationPromoterEFSAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybSEP2-WPRE
Plasmid#207661PurposeMonitoring neuropeptide releaseDepositorInsertCytochrome b-561 (Cyb561 Mouse)
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted 2 X SE…PromoterhSynapsinAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-shKcnk13
Plasmid#120721PurposeExpresses GFP and an shRNA targeting Kcnk13 (in pLL3.7)DepositorInsertKcnk13 (Kcnk13 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromotermouse U6Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kv7.1/pcDNA3.1
Plasmid#111452PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)
UseTags8xHis and EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-YY1
Plasmid#154943PurposeKnocking down of human YY1 through shRNA and amiRNA targeting YY1DepositorInsertshRNA and amiRNA against YY1 (YY1 Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCherryGFP-CoV2-Inframe-stop
Plasmid#177620PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).DepositorInsertSARS-CoV-2 FSE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaV1.2/pcDNA3.1~ Human
Plasmid#140576PurposeExpression in mammallian cells or oocytesDepositorInserthuman CaV1.2 (CACNA1C Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sRGECO
Plasmid#137125PurposeViral expression of sRGECODepositorHas ServiceAAV8InsertsRGECO
UseAAVTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 aSyn C141
Plasmid#108866PurposeExpresses human WT alpha synuclein with a C-terminal cysteineDepositorInsertalpha synuclein (SNCA Human)
UseTagsExpressionBacterialMutationC141 mutation to allow C-terminal maleimide dye l…PromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn GAPDH
Plasmid#220918PurposeNeuronal specific expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB1 (CK2alpha)
Plasmid#27083DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞MCS
Plasmid#154013PurposeExpression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoterDepositorInsertMCS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2w/b
Plasmid#111570PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorInsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-DIO-VGLUT2:miniSOG
Plasmid#214200PurposeFor Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)DepositorInsertSlc17a6 tagged with miniSOG and hemagglutinin
UseAAV and Cre/LoxTagsminiSOG (tagged with HA) is fused to the first in…ExpressionMammalianMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only