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pECE M2-SH3PXD2A WT

Plasmid

#69813

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA-mut

Plasmid

#140555

Purpose

Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

DA-insensitive sensor GRAB_DA-mut (control sensor)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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TC1316

Plasmid

#164883

Purpose

CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

dcas13d-ADARdd

Use

Tags

Expression

Mammalian

Mutation

dCas13d L560-G586 deleted

Promoter

CMV, T7

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B1-HA

Plasmid

#209209

Purpose

Mammalian expression of ATP8B1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B1 (ATP8B1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TC1681

Plasmid

#164872

Purpose

CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A fused to PspCas13b (APOBEC3A )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human apobec Y132D

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-KP1

Plasmid

#206504

Purpose

AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

KP1 capsid

Use

AAV

Tags

Expression

Mutation

Promoter

p5

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MSCVpic2neo-hTERT-p16 shRNA

Plasmid

#164920

Purpose

Expresses hTERT cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

human telomerase reverse transcriptase (TERT Human)
CDKN2A (CDKN2A Human)

Use

Retroviral

Tags

HA tag

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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YFVdelCME-mVenus

Plasmid

#179953

Purpose

pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking C, prM, E proteins but with mVenus

Use

Transneuronal tracing

Tags

Expression

Mutation

Promoter

SP6

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-BRD4ΔN-mCherry-sspB

Plasmid

#121968

Purpose

Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMXS-GshF-blasticidin

Plasmid

#210313

Purpose

Expresses humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide

Expression

Mutation

Codon optimized for expression in human cells

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_Short-IRES-eGFP

Plasmid

#196935

Purpose

Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

short isoform, lack of signaling casacade

Promoter

TRE

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TC1332

Plasmid

#164874

Purpose

CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A fused to PspCas13b (APOBEC3A )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human apobec Y132D

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-AC8(1-106)

Plasmid

#181958

Purpose

Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

EGFP-AC8(1-106) (Adcy8 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

Plasmid

#156448

Purpose

For transient expression of human CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human CTCF (CTCF Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (334-449)

Plasmid

#87859

Purpose

Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

fragment from aa 334 to 449

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-LoxP-PURO-LoxP-SNAP-TERT

Plasmid

#71390

Purpose

Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Use

Hr donor

Tags

FLAG-SNAP

Expression

Mutation

Promoter

Endogenous TERT promotor

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1

Plasmid

#213583

Purpose

Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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