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Using a barcoded AAV capsid library to select for clinically relevant gene therapy vectors.

Pekrun K, De Alencastro G, Luo QJ, Liu J, Kim Y, Nygaard S, Galivo F, Zhang F, Song R, Tiffany MR, Xu J, Hebrok M, Grompe M, Kay MA
JCI Insight. 2019 Nov 14;4(22). pii: 131610. doi: 10.1172/jci.insight.131610. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
83279pscAAV-CAG-GFPrecombinant AAV vector packaging self-complementary GFP under the CAG promoter
83281pAAV-CAG-FLucrecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)
206504AAV-KP1AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

Antibodies from Article