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Plasmid#185590Purposeto see protein dynamicsDepositorInsertVasa-E441Q
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationE441QPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MiniSOG-SpAGS-GFP
Plasmid#185581Purposefor CALIDepositorInsertminiSOG-SpAGS
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpAGS-dGoLoco1-GFP
Plasmid#185577Purposefor overexpressionDepositorInsertAGS-dGoLoco1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationdeletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-TetO-GFP_ColE1
Plasmid#171660PurposeQUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.DepositorInsertQUAS-5-T7-TetO-GFP ssra-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-TetO-GFP_ColE1
Plasmid#171662PurposeQUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.DepositorInsertQUAS-T7-TetO-GFP ssrA-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA5-mVenus
Plasmid#185646PurposeNDUFA5 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA5 (NDUFA5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFB6-mVenus
Plasmid#185652PurposeNDUFB6 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFB6 (NDUFB6 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFS3-mVenus
Plasmid#185649PurposeNDUFS3 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFS3 (NDUFS3 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shFmnl2-EGFP
Plasmid#187255PurposeLentiviral expression of shRNA targeting Fmnl2DepositorInsertShRNA for Fmnl2 (Fmnl2 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterU6Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-EGFP-HRasC20
Plasmid#187252PurposeLenticiral expression of EGFP-HRasC20DepositorInsertHRasC20 (HRAS Human)
UseLentiviralTagsEGFPExpressionMutationPromoter5' LTRAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pxyl-ftsZ-sfGFP
Plasmid#174505PurposeIntegrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose (sequence of sfGFP optimized)DepositorInsertftsZ
UseTagssuperfold GFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-VP16
Plasmid#183927PurposeOptogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposureDepositorUseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SUN1 GFP
Plasmid#187682PurposeIn vivo visualization of SUN1DepositorInsertSUN1 (Sun1 Mouse)
UseTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptia1l-hsp-GFP
Plasmid#177837PurposeDonor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9DepositorInserttia1l-sgRNA target site-hsp70-EGFP
UseCRISPRTagsEGFPExpressionMutationPromoterhsp70Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b motor
Plasmid#187364PurposeExpresses rat myosin 1b isoform b motor domain labelled with EGFPDepositorInsertmyosin 1b isoform b motor domain (Myo1b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-mGreenLantern/EGFP
Plasmid#188482PurposegRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.DepositorInsertmGreenLantern/EGFP sgRNA
UseCRISPRTagsExpressionMammalianMutationNonePromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-DRS2
Plasmid#177104PurposeExpresses iGFP-Drs2 in yeast cellsDepositorInsertDRS2 (DRS2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPaip1_G
Plasmid#146353PurposeInsect Expression of DmPaip1DepositorInsertDmPaip1
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only