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Addgene

pLL5.0-EGFP-HRasC20
(Plasmid #187252)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187252 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLL5.0
  • Backbone size w/o insert (bp) 6919
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HRasC20
  • Alt name
    CAAX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    795
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Promoter 5' LTR
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid made by Hiroko Kambe

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pLL5.0-EGFP was digested with EcoRI and SbfI to remove EGFP, and then EGFP-C20 fragment was inserted and ligated by In-Fusion cloning kit.

5' cloning site: EcoR1 (destroyed), 3' cloning site: Sbf1 (destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0-EGFP-HRasC20 was a gift from Alpha Yap (Addgene plasmid # 187252 ; http://n2t.net/addgene:187252 ; RRID:Addgene_187252)
  • For your References section:

    Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation. Katsuno-Kambe H, Teo JL, Ju RJ, Hudson J, Stehbens SJ, Yap AS. Elife. 2021 Oct 18;10. pii: 67915. doi: 10.7554/eLife.67915. 10.7554/eLife.67915 PubMed 34661524