pLL5.0-EGFP-HRasC20
(Plasmid
#187252)
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PurposeLenticiral expression of EGFP-HRasC20
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187252 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLL5.0
- Backbone size w/o insert (bp) 6919
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHRasC20
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Alt nameCAAX
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SpeciesH. sapiens (human)
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Insert Size (bp)795
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
- Promoter 5' LTR
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EGFP, forward primer (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmid made by Hiroko Kambe
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pLL5.0-EGFP was digested with EcoRI and SbfI to remove EGFP, and then EGFP-C20 fragment was inserted and ligated by In-Fusion cloning kit.
5' cloning site: EcoR1 (destroyed), 3' cloning site: Sbf1 (destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL5.0-EGFP-HRasC20 was a gift from Alpha Yap (Addgene plasmid # 187252 ; http://n2t.net/addgene:187252 ; RRID:Addgene_187252) -
For your References section:
Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation. Katsuno-Kambe H, Teo JL, Ju RJ, Hudson J, Stehbens SJ, Yap AS. Elife. 2021 Oct 18;10. pii: 67915. doi: 10.7554/eLife.67915. 10.7554/eLife.67915 PubMed 34661524