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Plasmid#182570PurposeHuman fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-MBP-TEV-Cas6-RT-Cas1_WT
Plasmid#186274PurposeExpresses Thiomicrospira Cas6-RT-Cas1 in E. coliDepositorInsertThiomicropira Cas6-RT-Cas1
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PlmCas12e
Plasmid#188496PurposeExpresses FLAG tagged PlmCas12eDepositorInsertCas12e
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-a-element-L4
Plasmid#186366PurposeEntry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequencesDepositorInserta-element early Otx neural enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3
Plasmid#73542PurposeExpression of sgRNA targeting LINE-1 and TagBFPDepositorInsertLINE-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1
Plasmid#73543PurposeExpression of sgRNA targeting LINE-1 and TagBFPDepositorInsertLINE-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-T11HA-hMOV10-CD
Plasmid#178908Purposeexpression of catalytic dead hMOV10 K530ADepositorInsertcDNA Catalytic dead hMOV10 (MOV10 Human)
UseTagsT11 beta strand of GFP-HAExpressionMammalianMutationK530A catalytic dead AAG>GCAPromoterCMV protmoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9341 (pgRNA_IX-1_NatMX)
Plasmid#161593PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9343 (pgRNA_XV-1_NatMX)
Plasmid#161595PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9358(VII-1_Markerfree_BackBone)
Plasmid#161599PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9359(VIII-1_Markerfree_BackBone)
Plasmid#161600PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9361(XIII-1_Markerfree_BackBone)
Plasmid#161602PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9363(XVI-1_Markerfree_BackBone)
Plasmid#161604PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppwd1 (PPWD1 Human)
UseTags(His)6-thrombinExpressionBacterialMutation473-646-W601APromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H
Plasmid#187188PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34R, D87RPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H
Plasmid#187184PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32F, L33FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H
Plasmid#187185PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90A, V91APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H
Plasmid#187183PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32A, L33APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox12-PLAT-EGFP-6H
Plasmid#187190PurposeProtein ExpressionDepositorInsertENSDARG00000069463 (alox12 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-PKCgamma-C2-EGFP-6H
Plasmid#187115PurposeProtein ExpressionDepositorInsertENSG00000126583 (PRKCG Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only