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Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas ServiceAAV5InserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Cre
Plasmid#170367PurposeAAV vector expressing Cre under human synapsin promotorDepositorInsertCre
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-MCS
Plasmid#185594PurposeControl lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11
Plasmid#211942Purposedual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHSP68Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-A KD/MD
Plasmid#104449PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein A (VAPA Human)
UseTagsEGFPExpressionMammalianMutationchanged K94 to D and M96 to DPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport SREBP-1c
Plasmid#8883DepositorInsertSREBP-1c (Srebf1 Mouse)
UseTagsExpressionMammalianMutationActive version of SREBP-1c.PromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPath-Violacein-SGE
Plasmid#200003PurposeViolacein pathway. SGEDepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIMAY*
Plasmid#121441PurposeAllelic exchange plasmid with PheS* counter-selection for Staphylococcus aureusDepositorTypeEmpty backboneUseEscherichia coli/staphylococcus aureus shuttle ve…TagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGY577
Plasmid#166663PurposeLight-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombinationDepositorInsertLiCre
UseCre/Lox and LentiviralTagsExpressionMutationCre: E340A, D341APromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorUseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-DVL1-WT
Plasmid#194586PurposeMammalian Expression of mEGFP-DVL1 WT Fusion ProteinDepositorInsertDVL1 (DVL1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_27F3_hFc
Plasmid#199619PurposeEncodes DARPin-hFc 27F3 for mammalian expression and column purificationDepositorInsertDARPin-hFc 27F3
UseTagshFcExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-ecfp
Plasmid#65030PurposeGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA mcherry
Plasmid#167910PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneUseTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialMutationPromoterT7-LacOAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E--3mnx1
Plasmid#74632PurposeCan be used to drive expression specifically in zebrafish motor neuronsDepositorInsertmotor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-tdTomato
Plasmid#133569PurposeCAG tdTomato piggyBac transposonDepositorInserttdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459V2.0_Conc2
Plasmid#134451PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + PuroDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_mCherry
Plasmid#182939Purposealpha7 nACHR expression in mammalian cell linesDepositorInsertNACH7 (CHRNA7 Human)
UseSynthesizedTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PKM2
Plasmid#64698PurposeExpression of GFP tagged PKM2 in mammalian cellsDepositorInsertPKM2 (PKM Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR
Plasmid#160046PurposeFor expressing the full paradoxical circuitDepositorInsertsosTIR1
iaaH
iCasp9
BlastR
UseSynthetic BiologyTagsmAID, mCherry, and mGFPmut3ExpressionMammalianMutationPromoterno promoter, T2A is used and pEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta into TRBC1 HDRT Source (pTR 277)
Plasmid#112024PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-betaDepositorInsertNYESO beta into TRBC1 HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR0306 CcaCas13b His6-TwinStrep-SUMO
Plasmid#182687PurposeExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purificationDepositorInsertCcaCas13b
UseTagsSUMOExpressionBacterialMutationPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only