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Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY1935
Plasmid#212315PurposeExpress ApmFnuc protein with sumotagDepositorInsertIsvmimi
UseTags6xHis-thrombin site-TwinStrepTag-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAIP-hXRCC1wt-eGFP
Plasmid#206032PurposeMammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoterDepositorInserthXRCC1
UseTagseGFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
XPO6-2Strep
Plasmid#210356PurposeExpresses XPO6 fused with 2Strep in mammalian cellsDepositorInsertXPO6 (XPO6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM2-2Strep
Plasmid#210374PurposeExpresses TRAM2 fused with 2Strep in mammalian cellsDepositorInsertTRAM2 (TRAM2 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM1-2Strep
Plasmid#210352PurposeExpresses TRAM1 fused with 2Strep in mammalian cellsDepositorInsertTRAM1 (TRAM1 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TMEM106B-2Strep
Plasmid#210360PurposeExpresses TMEM106B fused with 2Strep in mammalian cellsDepositorInsertTMEM106B (TMEM106B Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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SGTA-2Strep
Plasmid#210353PurposeExpresses SGTA fused with 2Strep in mammalian cellsDepositorInsertSGTA (SGTA Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG164
Plasmid#202583PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-hptIIDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 AvrRpm1
Plasmid#209185PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tagsDepositorInsertAvrRpm1
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80-421)msGFP
Plasmid#209014PurposeOverexpression of truncated syt1 tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 80-421 onlyPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE011
Plasmid#207912PurposeLevel 0 for gRNA in C4 module (5' GCAG-3' TCAG)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80 JuxtaK-142)
Plasmid#209013PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(143-421)msGFP
Plasmid#209010PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80 JuxtaK-421)
Plasmid#209008PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutationsDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(143-421)msGFP
Plasmid#209015PurposeOverexpression of truncated syt1 tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS018
Plasmid#207911PurposeLevel 0 for gRNA in C3 module (5' TTCG-3' GCAG)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only