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Addgene

pcDNA3.1-CRISPR-Puro-sgRPL3-62
(Plasmid #208647)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 208647 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1-CRISPR-Puro
  • Modifications to backbone
    refer to (Addgene#208646)
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418), Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    We use stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62
  • Alt name
    RPL3-sgR62
  • gRNA/shRNA sequence
    TCTTTCTTCAGTGGTCCCTG--/PAM/tGG/
  • Species
    H. sapiens (human), Synthetic
  • GenBank ID
    NC_000022.11:39312968-39312987
  • Entrez Gene
    RPL3 (a.k.a. ASC-1, L3, TARBP-B, uL3)
  • Promoter CMV and U6 in tandem

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI-v2 (destroyed during cloning)
  • 3′ cloning site BsmBI-v2 (destroyed during cloning)
  • 5′ sequencing primer CMV-F, Human-U6-promoter-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

co-transfect with pcDNA3.1-CRISPR-Puro-sgRPL3-56 (Addgene#208979) for better knock-in, single cut not perfermed good for knock-in.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CRISPR-Puro-sgRPL3-62 was a gift from Quanfu Ma (Addgene plasmid # 208647 ; http://n2t.net/addgene:208647 ; RRID:Addgene_208647)