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Showing: 6441 - 6460 of 14550 results

  2. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid
    #178236
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.VSVg.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178236-plasmid-map-sequence-id-352833

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  3. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid
    #178234
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178234-plasmid-map-sequence-id-352828

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  4. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80066
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80066-plasmid-map-sequence-id-152619

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  5. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid
    #178209
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.HA and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    178209-plasmid-map-sequence-id-352442

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  6. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  7. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid
    #178211
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    Nuclear Localization Signal and Ollas.S.V5
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178211-plasmid-map-sequence-id-367192

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  8. pLKO_C.HA.V5_mCherry-NLS

    Plasmid
    #178242
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178242-plasmid-map-sequence-id-352843

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  11. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

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  12. pBK092

    Plasmid
    #183146
    Purpose
    pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression
    Depositor
    Daan Swarts
    Article
    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.
    Insert
    CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
    Use
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    183146-plasmid-map-sequence-id-360089

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  14. pLCHKO_GFP_Luciferase

    Plasmid
    #155079
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155079-plasmid-map-sequence-id-308671

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  18. pDisplay-GRAB_CRF0.5

    Plasmid
    #208654
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208654-plasmid-map-sequence-id-410339

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  20. pLKO_NWS.Ollas.S_mCherry-NLS

    Plasmid
    #178279
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178279-plasmid-map-sequence-id-353143

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Showing: 6441 - 6460 of 14550 results