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Plasmid#148409PurposeInsect Expression of 5BoxB-polyA-HhRDepositorInsert5BoxB-polyA-HhR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-HsAsb9-3UTR_X
Plasmid#147949PurposeMammalian Expression of HsAsb9-3UTRDepositorInsertHsAsb9-3UTR (ASB9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X
Plasmid#147924PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat (Malat1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-Q123E_T
Plasmid#147555PurposeMammalian Expression of HsSmg5-Q123EDepositorInsertHsSmg5-Q123E (SMG5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsUPF2-siRNAres_I
Plasmid#146573PurposeMammalian Expression of HsUPF2-siRNAresDepositorInsertHsUPF2-siRNAres (UPF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-DmHunchback_W
Plasmid#147818PurposeInsect Expression of DmHunchback-3UTRDepositorInsertDmHunchback-3UTR (hb Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-tepsin
Plasmid#196707PurposeExpresses GFP-tepsin in mammalian cellsDepositorInserttepsin (TEPSIN Human)
UseTagsEGFPExpressionMammalianMutationsilent substitution in codon 199 to eliminate int…PromoterCMVAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A-apCC-Di
Plasmid#190706PurposeEncodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.DepositorInsertN-Venus-V150A-apCC-Di
UseTagsExpressionMutationV150APromoteraraBADAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-ASAP3ER
Plasmid#195358PurposeASAP3ER under control of human synapsin promoterDepositorInsertASAP3ER
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-eDHFR
Plasmid#194635PurposeMammalian expression of the eDHFR control bait in VirotrapDepositorInsertp55 GAG
UseTagsecDHFRExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo
Plasmid#191398PurposeHP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-NLS-MaSat ZF-18-mVenusN
Plasmid#191392PurposeZinc finger fused to N-terminal part of split mVenusDepositorInsertFlag-NLS-MaSat ZF-18-mVenusN
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAL1-VP1 (LEU2)
Plasmid#182480PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertdeltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRuby3 only VLP
Plasmid#182429PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-mRuby3
deltaVP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-eBra-L4
Plasmid#186368PurposeEntry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertBrachury notochord enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-Titf-L4
Plasmid#186367PurposeEntry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertTitf endoderm enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-pSnail-L5
Plasmid#186365PurposeEntry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertSnail regulatory sequence
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Full-length WT talin1
Plasmid#182992Purposeexpress FL WT talin1 in mammalian cellsDepositorInsertTalin1 WT (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only