pENT-L3-eBra-L4
(Plasmid
#186368)
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PurposeEntry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR-221-P3-P4
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Backbone manufacturerPMID: 17878951
- Backbone size w/o insert (bp) 2544
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Vector typeProtein expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBrachury notochord enhancer
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SpeciesC. intestinalis (ascidian)
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Insert Size (bp)405
- Promoter T7 promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13forward: TGTAAAACGACGGCCAGT
- 3′ sequencing primer M13rev: caggaaacagctatgac (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCiona eBra : https://www.aniseed.cnrs.fr/aniseed/gene/show_gene?unique_id=Phmamm.g00007005.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Ciona Brachyury enhancer drives expression in the early notochord lineages.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENT-L3-eBra-L4 was a gift from Patrick Lemaire (Addgene plasmid # 186368 ; http://n2t.net/addgene:186368 ; RRID:Addgene_186368) -
For your References section:
A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P. PLoS One. 2007. 2(9):e916. 10.1371/journal.pone.0000916 PubMed 17878951
