Addgene

We narrowed to 14,547 results for: LEA

Showing: 6361 - 6380 of 14547 results

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OCT4-eGFP-PGK-Puro

Plasmid

#31937

Purpose

Oct4 targeting donor vector

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

OCT4 (POU5F1 Human)

Use

Targeting donor

Tags

eGFP

Expression

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-NRBP1

Plasmid

#48197

Purpose

Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

Depositor

Ronald Conaway, Joan Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

NRBP1 (NRBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
EF.delmFasL.CMV.GFP

Plasmid

#17620

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Insert

delFasL (Fasl Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Noncleavable mouse delFasL . Deleted the exon 2 r…

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc1/bC

Plasmid

#74049

Purpose

retroviral expression plasmid for human NFATc1/bC

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

no tag

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-TetR-neoR

Plasmid

#153310

Purpose

Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Insert

TetR

Use

Transposon

Tags

Expression

Mutation

Promoter

SV40

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

Plasmid

#163572

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-UdgX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
pB-Tet-On-Arl13b-RhoA sensor

Plasmid

#189613

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

Arl13b

Use

Tags

GFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Tet3G

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway

Tags

3xFLAG

Expression

Mutation

Serine 127 changed to alanine

Promoter

none

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RR

Plasmid

#108860

Purpose

LbCpf1 Gateway entry plasmid with G548R and K611R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

LbCpf1-RR (LbCpf1 with G548R and K611R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

G548R and K611R, Cpf1 is rice codon optimized

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/FonEYFP

Plasmid

#55652

Purpose

Cre-off/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre off/flp on eyfp

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

Plasmid

#187578

Purpose

Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

WRAP53 (WRAP53 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-dL5**-mCER-TRF1

Plasmid

#168176

Purpose

Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

Depositor

Patricia Opresko

Article

Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

FAP (dL5**) and mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

Plasmid

#163562

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mTurquoise2

Plasmid

#169222

Purpose

Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDsRed-importinα

Plasmid

#119679

Purpose

Expresses DsRed-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

importinα (KPNA2 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Contains amino acids 251-529

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HS-GFP-PACT

Plasmid

#105954

Purpose

heat shock inducible transgenesis, PACT is a centriol marker

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PACT (AKAP9 Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#214052

Purpose

Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Target sequence

Use

Target rna plasmid

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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