pLVX-dL5**-mCER-TRF1
(Plasmid
#168176)
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PurposeExpresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLVX-IRES-Neo
- Total vector size (bp) 10904
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRF1
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SpeciesH. sapiens (human)
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Insert Size (bp)2634
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Entrez GeneTERF1 (a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
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Tag
/ Fusion Protein
- FAP (dL5**) and mCerulean (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-dL5**-mCER-TRF1 was a gift from Patricia Opresko (Addgene plasmid # 168176 ; http://n2t.net/addgene:168176 ; RRID:Addgene_168176) -
For your References section:
Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis. Fouquerel E, Barnes RP, Uttam S, Watkins SC, Bruchez MP, Opresko PL. Mol Cell. 2019 Jul 11;75(1):117-130.e6. doi: 10.1016/j.molcel.2019.04.024. Epub 2019 May 14. 10.1016/j.molcel.2019.04.024 PubMed 31101499
Map uploaded by the depositor.
