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pENTR1A mKate2-hu_ncOGT 5N5A

Plasmid

#154285

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Expression

Mutation

N322A, N356A, N390A, N424A, N458A mutations (HCF-…

Promoter

Availability

Academic Institutions and Nonprofits only

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KRAS-V14I

Plasmid

#154427

Purpose

V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2-CMV/TetO2-EYFP-GW

Plasmid

#153309

Purpose

Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Type

Empty backbone

Use

Transposon

Tags

Yellow Fluorescent Protein

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2-TetR-neoR

Plasmid

#153310

Purpose

Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Insert

TetR

Use

Transposon

Tags

Expression

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1-1089_K

Plasmid

#146804

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1085-1605_K

Plasmid

#146805

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1089_K

Plasmid

#146806

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1085-1605_K

Plasmid

#146807

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del1047-1063-GSG-dsRNAres_Y

Plasmid

#148070

Purpose

Insect Expression of DmPAN2-del1047-1063-GSG-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del1047-1063-GSG-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared the the sequence gei…

Promoter

Availability

Academic Institutions and Nonprofits only

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pLHCX-XBP1 mNeonGreen NLS

Plasmid

#115971

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Human, Synthetic)

Use

Retroviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RR

Plasmid

#108860

Purpose

LbCpf1 Gateway entry plasmid with G548R and K611R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

LbCpf1-RR (LbCpf1 with G548R and K611R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

G548R and K611R, Cpf1 is rice codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RVR

Plasmid

#108861

Purpose

LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

G548R, K554V and Y558R, Cpf1 is rice codon optimi…

Promoter

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RR

Plasmid

#108862

Purpose

FnCpf1 Gateway entry plasmid with N623R and K687R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RR (FnCpf1 with N623R and K687R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R and K687R, Cpf1 is rice codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

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pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

Plasmid

#46941

Purpose

Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

embedded TALEN TS from 163-218, truncated 25 amin…

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

Plasmid

#99743

Purpose

Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Use

Tags

myc-myc-his

Expression

Mammalian

Mutation

Changed both L165 and E166 to Alanine

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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proISG15 C-term. domain (79-165)

Plasmid

#110763

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. domain (79-157)

Plasmid

#110764

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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HS-GFP-PACT

Plasmid

#105954

Purpose

heat shock inducible transgenesis, PACT is a centriol marker

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PACT (AKAP9 Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)

Plasmid

#110143

Purpose

Transformation and expression of Csy4 and cogDsRed proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Binary vector

Tags

cogDsRed

Expression

Bacterial and Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)

Plasmid

#110144

Purpose

Transformation and expression of Csy4 and cogGFP proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Tags

cogGFP

Expression

Bacterial and Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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