We narrowed to 15,326 results for: LEA

1. pCMV_eA3A-NG-nCas9

   Plasmid

   #163536

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PD-1-miSFIT-Ce67

   Plasmid

   #124680

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pClneoMyc human MST2

   Plasmid

   #37022

   Depositor

   Yutaka Hata

   Article

   Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

   Insert

   MST2 (STK3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pHAGE2-TetOminiCMV-OS

   Plasmid

   #136576

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Use

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR366 RNF2

   Plasmid

   #47509

   Purpose

   human gRNA expression vector targeting RNF2

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-RNF2 (RNF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-TetOminiCMV-cMyc

   Plasmid

   #136614

   Purpose

   Dox-inducible lentiviral vector expressing mouse cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   cMyc (Myc Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CD81-(TEV)-FKBP-Myc

   Plasmid

   #177908

   Purpose

   human CD81-TEV cleavage site-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81 (CD81 Synthetic, Human)
   

   Use

   Tags

   FKBP, Myc tag, and TEV protease cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-G1-ATP8-Myc-FLAG (G418)

    Plasmid

    #86850

    Purpose

    mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Use

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX_317-C7orf26v1

    Plasmid

    #178537

    Purpose

    Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Insert

    C7orf26v1 (INTS15 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. shFGF2#2

    Plasmid

    #157668

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE-Flag-Cdk9-D167N-IRES-eGFP

    Plasmid

    #28098

    Depositor

    Andrew Rice

    Article

    Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains D167N mutation in Cdk9

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid

    #129394

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV and Cre/Lox

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. anxA10-GFP

    Plasmid

    #107199

    Purpose

    Expresses human annexin A10-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

    Insert

    annexin A10 (ANXA10 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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