We narrowed to 15,810 results for: LEA

1. pET-Cb2

   Plasmid

   #53529

   Purpose

   Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

   Depositor

   Gabor Igloi

   Article

   Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

   Insert

   mitochondrial arginyl-tRNA synthetase
   

   Tags

   His Tag and Thioredoxin

   Expression

   Bacterial

   Mutation

   changed Ser4 to Ala4 to permit NcoI cloning

   Promoter

   T7

   Available Since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. XE224 human flag-Chibby-pCS3+FLT

   Plasmid

   #16824

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   flag-Chibby (CBY1 Human)
   

   Use

   Xenopus expression

   Tags

   flag

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

3. MDH1-miR-196a-2SM-PGK-GFP-Blast

   Plasmid

   #31512

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-196a2 (Mir196a-2 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   seed mutant control

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

4. HOXB6-FLAGA-pSP65

   Plasmid

   #20994

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   In vitro trans/trans

   Tags

   FLAG

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA4-rB90

   Plasmid

   #18959

   Depositor

   Mariano Viapiano

   Article

   Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

   Insert

   Brevican C-terminus (Bcan Rat)
   

   Expression

   Mammalian

   Mutation

   Rat brevican C-terminus: Signal peptide aa 1-22 …

   Available Since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pDD2000

   Plasmid

   #101197

   Purpose

   Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
   

   Use

   Luciferase

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PKAsub(T/A)-RLuc8N-NES

   Plasmid

   #138212

   Purpose

   Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub(T/A)-RLuc8N-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFSW Doc2alpha D291N mutant

   Plasmid

   #128813

   Purpose

   To make virus expressing Doc2alpha D291 mutant

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Mutation

   D291N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFSW Doc2beta D303N mutant

   Plasmid

   #128815

   Purpose

   To make virus expressing Doc2beta D303N mutant

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Mutation

   D303N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFSW Doc2alpha Halotag

    Plasmid

    #128817

    Purpose

    To make virus expressing Doc2alpha Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW Doc2beta Halotag

    Plasmid

    #128818

    Purpose

    To make virus expressing Doc2beta Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZT-C13-R1

    Plasmid

    #62197

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Right CLYBL TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZT-C13-L1

    Plasmid

    #62196

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Left CLYBL TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid

    #208717

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Has Service

    AAV1, AAV2, and AAV8

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-NQL005-SOX2-sgRNA

    Plasmid

    #175553

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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