We narrowed to 15,832 results for: LEA

1. pFBD-Akt13C(WT)

   Plasmid

   #86576

   Purpose

   Expresses cleavable human full-length Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-AVITEV-GFP

   Plasmid

   #74052

   Purpose

   retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   AVITEV-GFP
   

   Use

   Retroviral

   Tags

   AVI-TEV-tag N-terminal to the GFP

   Expression

   Mammalian

   Promoter

   pMSCV-LTRs

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIA-CEN-FLAG-LINXa4-Bre1

   Plasmid

   #73617

   Purpose

   Control of the E3 ligase Bre1 with LINXa in yeast.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa4-Bre1dNLS
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A

   Promoter

   ADH1

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO myc Separase delta 55

   Plasmid

   #59824

   Purpose

   Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   delta 55

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Cb2

   Plasmid

   #53529

   Purpose

   Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

   Depositor

   Gabor Igloi

   Article

   Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

   Insert

   mitochondrial arginyl-tRNA synthetase
   

   Tags

   His Tag and Thioredoxin

   Expression

   Bacterial

   Mutation

   changed Ser4 to Ala4 to permit NcoI cloning

   Promoter

   T7

   Available Since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE224 human flag-Chibby-pCS3+FLT

   Plasmid

   #16824

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   flag-Chibby (CBY1 Human)
   

   Use

   Xenopus expression

   Tags

   flag

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. MDH1-miR-196a-2SM-PGK-GFP-Blast

   Plasmid

   #31512

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-196a2 (Mir196a-2 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   seed mutant control

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

8. HOXB6-FLAGA-pSP65

   Plasmid

   #20994

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   In vitro trans/trans

   Tags

   FLAG

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA4-rB90

   Plasmid

   #18959

   Depositor

   Mariano Viapiano

   Article

   Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

   Insert

   Brevican C-terminus (Bcan Rat)
   

   Expression

   Mammalian

   Mutation

   Rat brevican C-terminus: Signal peptide aa 1-22 …

   Available Since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pDD2000

    Plasmid

    #101197

    Purpose

    Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
    

    Use

    Luciferase

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW Doc2alpha D291N mutant

    Plasmid

    #128813

    Purpose

    To make virus expressing Doc2alpha D291 mutant

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Mutation

    D291N

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFSW Doc2beta D303N mutant

    Plasmid

    #128815

    Purpose

    To make virus expressing Doc2beta D303N mutant

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Mutation

    D303N

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFSW Doc2alpha Halotag

    Plasmid

    #128817

    Purpose

    To make virus expressing Doc2alpha Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFSW Doc2beta Halotag

    Plasmid

    #128818

    Purpose

    To make virus expressing Doc2beta Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid

    #138212

    Purpose

    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub(T/A)-RLuc8N-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDG1-PHP.eB

    Plasmid

    #220799

    Purpose

    AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    E4, E2A, VA, AAV2 rep and PHP.eB cap genes
    

    Use

    AAV; Aav helper vector

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-YE1-BE4max

    Plasmid

    #198546

    Purpose

    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpRY-YE1-BE4max

    Plasmid

    #198554

    Purpose

    Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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