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Showing: 1501 - 1550 of 4441 results

  1. hSLBP_mNG_FS_Donor

    Plasmid
    #191387
    Purpose
    Donor for C-terminal tagging of endogenous hSLBP
    Depositor
    Yannick Doyon
    Insert
    mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191387-plasmid-map-sequence-id-378113

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  2. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

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  4. pSOX2-donor

    Plasmid
    #176364
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176364-plasmid-map-sequence-id-347872

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  5. pOTX2-donor

    Plasmid
    #176358
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OTX2 (OTX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176358-plasmid-map-sequence-id-347862

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  6. pSTAT5B-donor

    Plasmid
    #176339
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    STAT5B (STAT5B Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176339-plasmid-map-sequence-id-347884

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  7. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid
    #169362
    Purpose
    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169362-plasmid-map-sequence-id-333207

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  8. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    alpha tubulin T. gondii
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

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  9. pXylP-HygR

    Plasmid
    #168131
    Purpose
    Expression of HygR from the xylose-inducible xylP promoter
    Depositor
    Michael Bromley
    Insert
    XylP-HygR
    Use
    Aspergillus expression
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    xylP
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    168131-plasmid-map-sequence-id-329723

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  10. pOSR1-donor

    Plasmid
    #176363
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OSR1 (OSR1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176363-plasmid-map-sequence-id-347404

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  11. pZNF627-donor

    Plasmid
    #176365
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF627 (ZNF627 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176365-plasmid-map-sequence-id-347863

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  12. pZNF608-donor

    Plasmid
    #176362
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF608 (ZNF608 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176362-plasmid-map-sequence-id-347402

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  13. pZNF79-donor

    Plasmid
    #176371
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF79 (ZNF79 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176371-plasmid-map-sequence-id-347399

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  14. pZBTB4-donor

    Plasmid
    #176374
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZBTB4 (ZBTB4 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176374-plasmid-map-sequence-id-347293

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  15. pSNAPC5-donor

    Plasmid
    #176341
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SNAPC5 (SNAPC5 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176341-plasmid-map-sequence-id-347185

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  16. pAW8-aid-spo5DSR-Turg1:kanMX6

    Plasmid
    #169358
    Purpose
    C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169358-plasmid-map-sequence-id-333930

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  17. pTSHZ1-donor

    Plasmid
    #132479
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    132479-plasmid-map-sequence-id-274870

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  18. pZNF449-donor

    Plasmid
    #132515
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF449 (ZNF449 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132515-plasmid-map-sequence-id-275207

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  19. pZNF8-donor

    Plasmid
    #136689
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF8 (ZNF8 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136689-plasmid-map-sequence-id-274397

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  21. pBV58

    Plasmid
    #204603
    Purpose
    ERG11-3X Minidegron-5X FLAG-HIS3MX6
    Depositor
    Scott Moye-Rowley
    Insert
    Candida glabrata ERG11 (ERG11 )
    Use
    Tags
    auxin-inducible degron
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204603-plasmid-map-sequence-id-403443

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  22. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

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  23. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Depositor
    Ulrich Koszinowski, Martin Messerle
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MIEPmouse CMV MIEP
    Available since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    85711-plasmid-map-sequence-id-177907

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  24. pSOX11-donor

    Plasmid
    #176360
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX11 (SOX11 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176360-plasmid-map-sequence-id-347860

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  25. pNPAS1-donor

    Plasmid
    #176346
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    NPAS1 (NPAS1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176346-plasmid-map-sequence-id-347888

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  26. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid
    #169355
    Purpose
    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169355-plasmid-map-sequence-id-333206

    Enlarge

  27. pSOX15-donor

    Plasmid
    #176356
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX15 (SOX15 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176356-plasmid-map-sequence-id-347874

    Enlarge

  28. pAW8-aid-V5-eDSR-Turg1:kanMX6

    Plasmid
    #169365
    Purpose
    C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    1XV5
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169365-plasmid-map-sequence-id-333205

    Enlarge

  29. pAW8-aid-5Myc-Turg1:kanMX6

    Plasmid
    #169356
    Purpose
    C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    5XMyc
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169356-plasmid-map-sequence-id-333204

    Enlarge

  30. pPRDM15-donor

    Plasmid
    #176373
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    PRDM15 (PRDM15 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176373-plasmid-map-sequence-id-347401

    Enlarge

  31. pZIC3-donor

    Plasmid
    #176361
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZIC3 (ZIC3 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176361-plasmid-map-sequence-id-347420

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  32. pTHAP12-donor

    Plasmid
    #176348
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    THAP12 (THAP12 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176348-plasmid-map-sequence-id-347267

    Enlarge

  33. pPOU6F1-donor

    Plasmid
    #176359
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    POU6F1 (POU6F1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176359-plasmid-map-sequence-id-347859

    Enlarge

  34. pZNF22-donor

    Plasmid
    #176349
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF22 (ZNF22 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176349-plasmid-map-sequence-id-347263

    Enlarge

  35. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

    Plasmid
    #169361
    Purpose
    C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    1XV5
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169361-plasmid-map-sequence-id-333201

    Enlarge

  36. pZNF624-donor

    Plasmid
    #132511
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF624 (ZNF624 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132511-plasmid-map-sequence-id-275217

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  37. pJK521

    Plasmid
    #72440
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Unspecified
    Tags
    Expression
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72440-plasmid-map-sequence-id-133778

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  38. pJK577

    Plasmid
    #72528
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72528-plasmid-map-sequence-id-132616

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  40. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid
    #219828
    Purpose
    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    219828-plasmid-map-sequence-id-441125

    Enlarge

  41. AbVec2.0-mIghg2c

    Plasmid
    #127159
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg2c (Ighg2c Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127159-plasmid-map-sequence-id-256903

    Enlarge

  44. pINER5

    Plasmid
    #185895
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185895-plasmid-map-sequence-id-370388

    Enlarge

  45. pRARB-donor

    Plasmid
    #132517
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    132517-plasmid-map-sequence-id-274801

    Enlarge

  46. AbVec2.0-mIghg3

    Plasmid
    #127160
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg3 (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127160-plasmid-map-sequence-id-256904

    Enlarge

  47. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

    Enlarge

  48. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

    Enlarge

  49. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

    Enlarge

  50. pINER2R

    Plasmid
    #185882
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185882-plasmid-map-sequence-id-370829

    Enlarge

Showing: 1501 - 1550 of 4441 results