hSLBP_mNG_FS_Donor
(Plasmid #191387)

• Purpose: Donor for C-terminal tagging of endogenous hSLBP
• Depositing Lab: Yannick Doyon
• Publication: S-phase specific probe (unpublished)

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2364
• Total vector size (bp): 4250
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 885
• Entrez Gene: SLBP (a.k.a. HBP)

Resource Information

• Supplemental Documents:
  • Donneur SLBP_mNG_FS (3'UTR).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

-Use in combination with eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA (Plasmid #191528)

-See Marker-free coselection for CRISPR-driven genome editing in human cells. Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. 10.1038/nmeth.4265 PubMed 28417998