pLinker-6xHA-HXGPRT-LoxP
(Plasmid
#86552)
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PurposeThe plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2830
- Total vector size (bp) 2830
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Vector typeExpression in Toxoplasma gondii
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Selectable markersHXGPRT for Toxoplamsa gondii
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLinker-6xHA-HGPRT-LoxP
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SpeciesToxoplasma gondii
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer ttcgggcccgctagcAAGGGCTCGGGCTCGACCCAGCTGTACCCATACGATGTTCC
- 3′ sequencing primer TCGACCCTCGAGTAGAACTAGTGGATC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLinker-6xHA-HXGPRT-LoxP was a gift from David Sibley (Addgene plasmid # 86552 ; http://n2t.net/addgene:86552 ; RRID:Addgene_86552)