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mCherry-miniSOG-Keratin-17

Plasmid

#55091

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pWZL hygro Flag HA TRAP220 wt

Plasmid

#17433

Purpose

Retroviral expression of Med1/TRAP220 in mammalian cells

Depositor

Kai Ge

Article

Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Insert

TRAP220 (MED1 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Expression

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMIG-IRF4

Plasmid

#58987

Purpose

MSCV based expression vector - co-expresses GFP and murine IRF4

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Insert

Mouse IRF4 (Irf4 Mouse)

Use

Retroviral

Tags

Kozak sequence added

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.kc1-HSD17B10

Plasmid

#164076

Purpose

Human codon optimized HSD17B10. Expression is controlled by doxycycline.

Depositor

David Sabatini

Article

Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

HSD17B10 (HSD17B10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11

Plasmid

#140941

Purpose

expresses PTPN11

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

Plasmid

#204189

Purpose

Doxycycline inducible lentiviral vector for human GATA6 overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

Plasmid

#184398

Purpose

Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

del52

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Mito-7

Plasmid

#57795

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Golgi-7

Plasmid

#57791

Purpose

Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Golgi (B4GALT1 Human)

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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hu-TLR4 299/399 dual snp flag pDEST40

Plasmid

#42648

Purpose

destination vector for expression of human TLR4 299/399 SNP in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 299/399 SNP (TLR4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

D299G and T399I

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
pLX307/PTPN11 T73I

Plasmid

#140939

Purpose

expresses PTPN11 T73I

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

T73I

Promoter

EF1a

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 E76G

Plasmid

#140862

Purpose

expresses PTPN11 E76G

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

E76G

Promoter

EF1a

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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optoFcR

Plasmid

#224291

Purpose

Expression of an optogenetic Fc Receptor

Depositor

Meghan Morrissey

Article

Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

Insert

Fc receptor gamma (Fcer1g Mouse)

Use

Lentiviral

Tags

mScarlet-Cry2-olig and myristoylation sequence

Expression

Mutation

aa 45-86 only

Promoter

SFFV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR del52

Plasmid

#214768

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR del52 (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR ins5

Plasmid

#214769

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR ins5 (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-FLAG

Plasmid

#204524

Purpose

Retroviral expression of human CALR ins5-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

ins5

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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