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pLX307/PTPN11 T73I
(Plasmid #140939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX307
  • Backbone size w/o insert (bp) 10065
  • Total vector size (bp) 10500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ]
  • Alt name
    CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Mutation
    T73I
  • GenBank ID
    NM_002834
  • Entrez Gene
    PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
  • Promoter EF1a
  • Tag / Fusion Protein
    • V5

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX307/PTPN11 T73I was a gift from Kimberly Stegmaier (Addgene plasmid # 140939 ; http://n2t.net/addgene:140939 ; RRID:Addgene_140939)

  • For your References section:

    Resistance Mechanisms to SYK Inhibition in Acute Myeloid Leukemia. Cremer A, Ellegast JM, Alexe G, Frank ES, Ross L, Chu SH, Pikman Y, Robichaud A, Goodale A, Haupl B, Mohr S, Rao AV, Walker AR, Blachly JS, Piccioni F, Armstrong SA, Byrd JC, Oellerich T, Stegmaier K. Cancer Discov. 2020 Feb;10(2):214-231. doi: 10.1158/2159-8290.CD-19-0209. Epub 2019 Nov 26. 10.1158/2159-8290.CD-19-0209 PubMed 31771968
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