pMSCV-IRES-mOrange/CALR del52
(Plasmid
#214768)
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PurposeMammalian expression of human CALR del52
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMSCV-IRES-mOrange
- Backbone size w/o insert (bp) 6500
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Modifications to backbonemodify pMSCV-IRES-GFP(replace GFP to mOrange)
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman CALR del52
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SpeciesH. sapiens (human)
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Insert Size (bp)5496
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Entrez GeneCALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
- Promoter MSCV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer original (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-IRES-mOrange/CALR del52 was a gift from Norio Komatsu (Addgene plasmid # 214768 ; http://n2t.net/addgene:214768 ; RRID:Addgene_214768)