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Plasmid#79590PurposeTransient expression and retroviral ExpressionDepositorInsertOrai1 (ORAI1 Human)
UseRetroviralTagsFLAG (inserted between TM3 and TM4 of the protein…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-API5
Plasmid#157655PurposeExpresses API5 in E.coli cellDepositorInsertApoptosis inhibitor 5 (API5 Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10 Dub3
Plasmid#72684Purposeluciferase reporter with Dub3 promoterDepositorInsertDub3 promoter (Usp17le Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterDub3Available sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1
Plasmid#176838PurposeTo induce human PDX1 expression by recruiting SAM complex to PDX1 promoterDepositorInsertsgPDX1
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSN69
Plasmid#100125Purposeexpresses putative cMYC DBD with a HA tagDepositorInsertmCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
UseLentiviralTagsExpressionMammalianMutationInsert corresponds to the DBD of cMYCPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_APOBEC1-UdgX-NG-nCas9
Plasmid#163533PurposeMammalian CG-to-GC base editingDepositorInsertAPOBEC1-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DNM2-W525L
Plasmid#155263PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationTryptophan 525 to LeucinePromoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DNM2-R465W
Plasmid#155262PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationArginine 465 to TryptophanPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-NQL007-NANOG-sgRNA
Plasmid#175555PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.DepositorInsertspCas9-nuclease and sgRNA against mouse NANOG STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T12A
Plasmid#47936Purposeexpresses Myc tagged human MOB1 containing T12A mutationDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT12APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2
Plasmid#85667PurposeExpression of human YOD1 I292Q V295Q mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationI292Q and V295QPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shEsco2
Plasmid#160957PurposeEsco2 shRNA in pMKO.1 retroviral vectorDepositorInsertEsco2 shRNA (Esco2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Emerin
Plasmid#120246PurposeExpresses V5-tagged Emerin in lentiviral vectorDepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shBub1
Plasmid#160948PurposeBub1 shRNA in pMKO.1 retroviral vectorDepositorInsertBub1 shRNA (Bub1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-ER-E2F2-dRb
Plasmid#62805Purposeexpresses an inducible E2F2 mutant that lacks the Rb binding domainDepositorInsertE2F2 (E2F2 Human)
UseRetroviralTagsHA-ErExpressionMammalianMutationdeletion of amino acids 302-437, including the Rb…PromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBB0323-TEV-His12
Plasmid#137039Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66700.1
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin
Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB1 (human) HIS-tag pET
Plasmid#8520DepositorInsertHOXB1 (HOXB1 Human)
UseTagsHis and T7ExpressionBacterialMutationPCR from cDNA clonePromoterAvailable sinceJuly 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-N
Plasmid#27418DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTags2xFlagExpressionMammalianMutationDeletion of the carboxy-terminus of ZO-2, so only…PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only