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Plasmid#27323DepositorInsertubiquitin:EGFP reporter
UseTol2 transposonTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 epsilon
Plasmid#116886PurposeExpresses HA-tagged human 14-3-3 epsilon in mammalian cellsDepositorInsert14-3-3 epsilon (YWHAE Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Runx1
Plasmid#131609Purposeexpression of mouse Runx1 in mammalian cellsDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 (pCMV-IRES-GFP version 3)
Plasmid#78264PurposeLentiviral empty cloning vector containing IRES and GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsIRES-EGFPExpressionMammalianMutationPromoterCMV, lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR357.24
Plasmid#125109PurposeExpression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UAS-CITRINE
Plasmid#104839PurposeSPARK reporter geneDepositorInsertCitrine
UseAAVTagsExpressionMammalianMutationPromoterUASAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG SARS-CoV2 RdRp.co
Plasmid#154008PurposeExpression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cellsDepositorInsertCodon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
UseTags3xFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiGuide-Puro PRMT5-CRISPRi
Plasmid#164637PurposelentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2DepositorInserttarget guide sequence PRMT5 inhibition (PRMT5 Human)
UseCRISPR and Lentiviral; InterferenceTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPguCas13b-3xmRuby3-NLS
Plasmid#132406Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsExpressionMammalianMutationH151A; H1121APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
UseTagsExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Orai1-GCaMP6f
Plasmid#73564PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-GCaMP6f (ORAI1 Synthetic, Human)
UseTagsGCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TSC2
Plasmid#15478DepositorInsertTSC2 shRNA (TSC2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD
Plasmid#98669PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43DepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab8a-WT
Plasmid#101048PurposeExpresses HA-tagged human Rab8-WT in mammalian cellsDepositorInsertRab8a (RAB8A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SMN2E7-Fluc-Nluc-reporter
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_526
Plasmid#184859PurposeLentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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TFORF0985
Plasmid#142249PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HPV16 E6 TCR
Plasmid#122727PurposeExpress HPV16 E6 TCRDepositorInsertHPV16 E6 TCR
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9-MS2-BB_BbsI
Plasmid#164802PurposeREDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV-MyoD
Plasmid#26808DepositorInsertMyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -