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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2A2
- Backbone size w/o insert (bp) 4179
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Vector typeTol2 transposon
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameubiquitin:EGFP reporter
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Alt nameubi:EGFP
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Alt nameubi-EGFP
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Alt nameubi-GFP
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)4606
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site attB4 (not destroyed)
- 3′ cloning site attB3 (not destroyed)
- 5′ sequencing primer attB4
- 3′ sequencing primer attB3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are a few nucleotide discrepancies between author sequence and Addgene quality control sequence. According to the depositing lab, these do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDestTol2pA2_ubi:EGFP was a gift from Leonard Zon (Addgene plasmid # 27323 ; http://n2t.net/addgene:27323 ; RRID:Addgene_27323) -
For your References section:
Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish. Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI. Development. 2011 Jan . 138(1):169-77. 10.1242/dev.059345 PubMed 21138979