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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR5' TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2692
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Vector typeMultisite Gateway 5' entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameubiquitin:loxP-EGFP-loxP
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Alt nameubi:Switch
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Alt nameubi:loxP-EGFP-loxP
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)5855
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attL4 (not destroyed)
- 3′ cloning site attR1 (not destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR5'_ubi:loxP-EGFP-loxP was a gift from Leonard Zon (Addgene plasmid # 27322 ; http://n2t.net/addgene:27322 ; RRID:Addgene_27322) -
For your References section:
Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish. Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI. Development. 2011 Jan . 138(1):169-77. 10.1242/dev.059345 PubMed 21138979