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Showing: 1161 - 1200 of 156570 results

  1. pLenti-PH-PLC-delta-EGFP

    Plasmid
    #136998
    Purpose
    Lentiviral EGFP lipid sensor for PI(4,5)P2
    Depositor
    Ken-Ichi Takemaru
    Insert
    PH-PLC-delta-EGFP
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136998-plasmid-map-sequence-id-291838

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  10. phage UbiC NLS HA stdMCP stdHalo

    Plasmid
    #104999
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    stdMCP-stdHalo
    Use
    Lentiviral
    Tags
    HA tag, NLS, Two synonymous copies of the Halo-ta…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbiC
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104999-plasmid-map-sequence-id-202896

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  12. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55005-plasmid-map-sequence-id-456544

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  22. MR015-H11-L2-TALEN

    Plasmid
    #51554
    Purpose
    Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    H11-L2 TALEN
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51554-plasmid-map-sequence-id-78782

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  23. MR015-H11-R2-TALEN

    Plasmid
    #51555
    Purpose
    Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    H11-R2 TALEN
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51555-plasmid-map-sequence-id-78783

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  26. pM2s2AsG

    Plasmid
    #137924
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137924-plasmid-map-sequence-id-331367

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  27. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid
    #212020
    Purpose
    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    UtrCH
    Use
    Tags
    mStayGold(c4)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212020-plasmid-map-sequence-id-419202

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  28. p2attNG-H11-short

    Plasmid
    #51546
    Purpose
    p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    GFP4m
    Use
    Human targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51546-plasmid-map-sequence-id-78774

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  32. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  33. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    David Sabatini, Eric Lander
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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  35. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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Showing: 1161 - 1200 of 156570 results