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PurposeRetroviral expression of mouse Oct3/4
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePOU domain, class 5, transcription factor 1
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Alt nameOct3/4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1134
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MutationNone
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Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Mouse Oct3/4 includes C91T mutation.
We found that EST clones including substitution C91 to T in NCBI database. So we believe that these substitutions are ascribed to SNPs.
CJ069184 RIKEN full-length enriched mouse cDNA library, CRL-2070
CJ069414 RIKEN full-length enriched mouse cDNA library, CRL-2070
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-Oct3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 13366 ; http://n2t.net/addgene:13366 ; RRID:Addgene_13366) -
For your References section:
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174