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Plasmid#201794PurposeExpresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483EPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483F
Plasmid#201795PurposeExpresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483FPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483A
Plasmid#201796PurposeExpresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483APromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 WT
Plasmid#201797PurposeExpresses wild-type Tensin-2 protein in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-GFP
Plasmid#82506PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMygro
Plasmid#82503PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInserteGFP-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mCherry-IRESMygro
Plasmid#82505PurposeVector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInsertmCherry-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagBFP2-IRESMuro
Plasmid#82335PurposeVector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagBFP2-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagtdTom_IRESMuro
Plasmid#82355PurposeVector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagTandemTomato-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMuro
Plasmid#82504PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInserteGFP-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-Luc2-IRESMeo
Plasmid#82509PurposeVector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.DepositorInsertLuc2-IRESMeo
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-LacZ-IRESMuro
Plasmid#82507PurposeVector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertLacZ-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-Clover-Muro
Plasmid#82334PurposeVector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertClover-T2AMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMeo
Plasmid#82502PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.DepositorInserteGFP-IRESMeo
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
vc241
Plasmid#193453PurposeExpression of rRFP in A. castellaniiDepositorInsertmRFP
UseAcanthamoeba exressionTagsExpressionMutationPromoterGAPDHAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
vHB8
Plasmid#193454PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInsertGFP-Cas9-NLS
UseCRISPRTagsGFPExpressionMutationPromoterGAPDHAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSETT4gap
Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotergapDH promoter from Eggerthella lentaAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
UseTagsExpressionBacterial and YeastMutationPromoterGAL1 GAPDHAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only