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Showing: 101 - 150 of 358 results

  3. pET30-T7-sfGFP

    Plasmid
    #180757
    Purpose
    pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator
    Depositor
    Michael Black
    Article
    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:
    Insert
    superfolder green fluorescent protein
    Use
    Tags
    strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    180757-plasmid-map-sequence-id-359679

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  11. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Use
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

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  27. pIBA3-SpyCatcher-sfGFP

    Plasmid
    #107420
    Purpose
    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Use
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    Promoter
    Tet
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107420-plasmid-map-sequence-id-207599

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  28. pQE81XN-mGold

    Plasmid
    #218959
    Purpose
    Expresses Hisx6-Tagged mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    mGold
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218959-plasmid-map-sequence-id-445675

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  29. proinsulin-mGold

    Plasmid
    #218953
    Purpose
    Expresses proinsulin fused to mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-mGold
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218953-plasmid-map-sequence-id-446158

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  30. pQE81XN-msYFP

    Plasmid
    #218960
    Purpose
    Expresses Hisx6-Tagged msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218960-plasmid-map-sequence-id-446223

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  31. pQE81XN-msYFP2

    Plasmid
    #218961
    Purpose
    Expresses Hisx6-Tagged msYFP2 in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP2
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218961-plasmid-map-sequence-id-446222

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  32. proinsulin-msYFP2

    Plasmid
    #218955
    Purpose
    Expresses proinsulin fused to msYFP2 in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-msYFP2
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218955-plasmid-map-sequence-id-446161

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  33. proinsulin-EYFP

    Plasmid
    #218952
    Purpose
    Expresses proinsulin fused to EYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-EYFP
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218952-plasmid-map-sequence-id-446160

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  34. pQE81XN-EYFP

    Plasmid
    #218958
    Purpose
    Expresses Hisx6-Tagged EYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    EYFP
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218958-plasmid-map-sequence-id-445670

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  35. MFVN-msYFP

    Plasmid
    #218971
    Purpose
    Expresses the first 4 aa of proinsulin fused to msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    MFVN-msYFP
    Use
    Tags
    msYFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218971-plasmid-map-sequence-id-445646

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  36. pEN-R2-mCsfGFP-L3

    Plasmid
    #178602
    Purpose
    It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.
    Depositor
    Frederica Theodoulou
    Article
    Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.
    Insert
    Tandem fluorescent protein timers (tFTs)
    Use
    Entry vector for gateway cloning
    Tags
    mCherry superfolder GFP
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    178602-plasmid-map-sequence-id-350720

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  39. MFVN-mStayGold(J)

    Plasmid
    #225444
    Purpose
    Expresses mStayGold(J) fused to the first 4 amino acids of proinsulin
    Depositor
    Benjamin Glick
    Insert
    MFVN-mStayGold(J)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225444-plasmid-map-sequence-id-446224

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  41. pJL1-sfGFP-DQNAT

    Plasmid
    #198210
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198210-plasmid-map-sequence-id-393676

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  43. prCAG-RBS"m1"-sfGFP

    Plasmid
    #191400
    Purpose
    Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)
    Depositor
    Ariel Lindner
    Article
    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.
    Insert
    47xCAG RBS"m1"-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191400-plasmid-map-sequence-id-382526

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  44. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Use
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107421-plasmid-map-sequence-id-207622

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  47. pQE81XN-mStayGold(J)

    Plasmid
    #225445
    Purpose
    Expresses Hisx6-Tagged mStayGold(J) in bacteria
    Depositor
    Benjamin Glick
    Insert
    mStayGold(J)
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225445-plasmid-map-sequence-id-446225

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  48. pRMC2-msfGFP

    Plasmid
    #194913
    Purpose
    Tetracycline inducible expression of msfGFP in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194913-plasmid-map-sequence-id-384624

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  49. Cx26-msfGFP

    Plasmid
    #69016
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx26 (Gjb2 Rat)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69016-plasmid-map-sequence-id-124712

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  50. Cx43K258stop-msfGFP

    Plasmid
    #69023
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    delete codons 258-381 and fuse in frame to monome…
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69023-plasmid-map-sequence-id-124708

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Showing: 101 - 150 of 358 results