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Plasmid#200635PurposeNegative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cellsDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(S536E)
Plasmid#24156DepositorInsertRelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationS536EPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-His6
Plasmid#51536PurposeExpress His6-tagged mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
miR-vector-RFP pCDNA
Plasmid#97143Purposefor expression of miRNA and TagRFPDepositorTypeEmpty backboneUseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP73_sgRNA-1
Plasmid#88855PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSin wPGK-Cre
Plasmid#101242Purposelow-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expressionDepositorInsertCre recombinase
UseLentiviralTagsExpressionMutationsee attached filePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 H83L H86L
Plasmid#12211DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPBD (p21-binding domain) mutant: H83L, H86L (see …PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-2.4-VP64
Plasmid#103039PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activatorDepositorInsertTAL-L1-2.4-VP64
UseT3 in vitro transcription for expression in mamma…TagsHA and VP64ExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pALPS tat-P2A-rev
Plasmid#101331PurposeLentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)DepositorInsertNL4-3 tat and rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PDZD8HA_sh_resistant
Plasmid#105006PurposeMammalian Expression of PDZD8, sh resistantDepositorInsertPDZD8 (Pdzd8 Mouse)
UseTagsHAExpressionMammalianMutationThe sequence recognized by shPDZD8 (TRCN000043105…PromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Myo1b
Plasmid#187366PurposeExpresses rat myosin 1b isoform b domain labelled with CherryDepositorInsertmyosin 1b isoform b (Myo1b Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CIBN-CreC
Plasmid#75267PurposeThe first half of the photo-activatable cre recombinaseDepositorInsertN-terminal portion of the CIB1 transcription factor
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I delta isoform
Plasmid#22188DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I-p70
Plasmid#22187DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationContains only amino acids 1-735 C-terminal residu…PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal80ts
Plasmid#51619Purposevertebrate expression of full-length temperature-sensitive Gal80 with stopDepositorInsertGal80ts (GAL80 Budding Yeast)
UseVertebrate expressionTagsExpressionMutationtemperature sensitive (G203V, G323R)*PromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
4xHRE-MinTK-CRE
Plasmid#141144Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsert4xHRE-MinTK-CRE
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only