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Plasmid#90214PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only
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pOTTC475 - pAAV c-fos iRFP
Plasmid#47906PurposeAn AAV vector that expresses iRFP under the c-fos promoter.DepositorInsertInfrared fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterc-fosAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV pmSyn1-EBFP-Cre
Plasmid#51507PurposeCan be used to generate AAV virus that will express Cre in neurons from the synapsin promoterDepositorHas ServiceAAV5InsertEBFP-Cre
UseAAVTagsExpressionMutationPromoterpmSyn1Available sinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
VGlut1 scFv [N28/9]
Plasmid#182084PurposeMammalian Expression of VGlut1 scFv. Derived from hybridoma N28/9.DepositorInsertrecombinant mouse scFv targeting VGlut1 (Rattus norvegicus) (Slc17a7 Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
VGlut2 scFv [N29/29]
Plasmid#182085PurposeMammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.DepositorInsertrecombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
Plasmid#154001PurposeVector expressed CasRx and sgRNA_Ptbp1 for AAV packageDepositorInsertCasRx, Ptbp1 sgRNAs
UseCRISPR and Mouse TargetingTagsFLAGExpressionMutationPromoterGFAP, U6Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP313-pAAV-CMV-SaCas9-DIO-pA
Plasmid#113690PurposeA CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9 (NEWENTRY )
UseAAV, CRISPR, and Cre/LoxTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-tdTomato
Plasmid#62516PurposeExpresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing proceduresDepositorInserttdTomato
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_-DIO-GRAB_NPY1.0
Plasmid#208677PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent mannerDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseAAVTagsExpressionMutationPromoterEF1_Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC589 - pAAV c-fos Nuc-eYFP
Plasmid#59133PurposeAn AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.DepositorInsertNuclear-localized Enhanced Yellow Fluorescent Protein
UseAAVTags3xNLSExpressionMammalianMutationPromotermFosAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Fxr1
Plasmid#112732PurposeAAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoterDepositorInsertFxr1p (Fxr1 Mouse)
UseAAVTagsGFPExpressionMammalianMutationPromoterhSYNAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC364 - pAAV CaMKIIa iRFP
Plasmid#47903PurposeAn AAV vector that expresses iRFP under the CaM Kinase IIa promoter.DepositorInsertInfrared fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC337 - pAAV EF1a DIO mCherry
Plasmid#47636PurposeAn AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorInsertmCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC588 - pAAV c-fos Nuc-iRFP
Plasmid#59132PurposeAn AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.DepositorInsertNuclear-localized Infrared Fluorescent Protein
UseAAVTags3xNLSExpressionMammalianMutationPromotermFosAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-53BP1(1-1710) 3LA
Plasmid#185698PurposeMammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.DepositorInsert53BP1(1-1710) 3LA (TP53BP1 Human)
UseRetroviralTagsFlag tag, HA tag, and tetracysteine tagExpressionMammalianMutationL175A, L517A, and L693APromoterSV40Available sinceJuly 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMX-53BP1(1-1710) 3LA7A
Plasmid#185707PurposeMammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.DepositorInsert53BP1(1-1710) 3LA7A (TP53BP1 Human)
UseRetroviralTagsFlag tag, HA tag, and tetracysteine tagExpressionMammalianMutationT302A, S452A, S523A, S543A, S625A, S784A, and S89…PromoterSV40Available sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EFS-DIO-GRAB_NTS1.0
Plasmid#208671PurposeExpresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent mannerDepositorInsertGPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA
Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA
Plasmid#113685PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.DepositorInsertinverted de-catalyzed SaCas9
UseAAV, CRISPR, Cre/Lox, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 3
Plasmid#51762PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only